In silico cloning, expression and bioinformatics analysis of NtODB from common tobacco / 生物工程学报

It has been reported that ODB genes play an important role in homologous recombination-directed DNA repair, suggesting their potential applications in plant breeding. To analyze the expression characteristics of tobacco NtODB gene, the cDNA sequence of NtODB was obtained using in silico cloning technique. The physicochemical properties, signal peptide, and advanced structures of the predicted protein were analyzed using bioinformatics tools. The results showed that the NtODB gene has a 579-bp open reading frame which encodes a protein with 192 amino acid residues. The protein NtODB is predicted to be alkaline and hydrophilic. Real-time quantitative PCR showed that NtODB was constitutively expressed in different tissues. Subcellular localization showed that NtODB was mainly expressed in cell membrane and chloroplast. These results may help us to better understand and elucidate the roles of ODB genes in the homologous recombination-directed DNA repair.

Comparison of clinical efficacy of sphincter preservation seton and fistula resection in the treatment of com-plex anal fistulas / 中国基层医药

Objective To investigate the clinical efficacy of sphincter preservation seton and fistula resection in the treatment of patients with complex anal fistula.Methods According to the stochastic indicator method, 96 patients with complex anal fistula admitted in department of anus bowel in our hospital from April 2013 to July 2015 were divided into control group and research group,48 patients in each group.The control group underwent tradi-tional fistula resection,while the research group underwent sphincter preservation seton surgery.The short -term and long -term efficacy,operation time,length of hospital stay,wound healing time,wound area and anal function were analyzed statistically.All patients were followed up more than half a year after surgery,and the incidence of complica-tions and the recurrence rate were recorded in detail.Results The short -term cure rate of two groups was 100.0%, with no statistical differences (P >0.05).While the long -term cure rate of the research group was 93.8%,which was significantly higher than 81.3% of the control group(χ2 =3.851,P =0.032).The operation time,length of hospital stay,wound healing time and wound area of the research group were significantly lower than the control group (t =4.417,6.496,2.693,3.944,P =0.025,0.017,0.041,0.028).Compared with before treatment,the anal func-tion scores of the two groups after treatment were significantly decreased(t =12.269,22.085,P =0.009,0.005),and the anal function score of the research group was significantly lower than the control group(t =3.155,P =0.035). The incidence rate of complications of the research group was 10.4%(5 /48),which was significantly lower than 22.9%(11 /48)of the control group(χ2 =4.510,P =0.044).Conclusion Compared with traditional fistula resec-tion,sphincter preservation seton has better long -term clinical efficacy in treatment of complex anal fistula,which can effectively improve the surgery indexes and prognosis,and significantly reduce the incidence of postoperative complica-tions.It is worth popularizing in clinic widely.